アガートラップ法

AgarTrap methods

Easy genetic transformation methods for the liverwort Marchantia polymorpha L.

♣S-AgarTrap for sporelings
Tsuboyama S, Kodama Y (2014) AgarTrap: a simplified Agrobacterium-mediated transformation method for sporelings of the liverwort Marchantia polymorpha L. Plant and Cell Physiology, 55:229-236.

♣G-AgarTrap for intact gemmae/gemmalings

Tsuboyama-Tanaka S, Kodama Y (2015) AgarTrap-mediated genetic transformation using intact gemmae/gemmalings of the liverwort Marchantia polymorpha L. Journal of Plant Research, 128:337-344.

Tsuboyama S, Nonaka S, Ezura H, Kodama Y (2018) Improved G-AgarTrap: A highly efficient transformation method for intact gemmalings of the liverwort Marchantia polymorpha. Scientific Reports, 8:10800.

Tsuboyama S, Kodama Y (2018) Highly efficient G-AgarTrap-mediated transformation of the Marchantia polymorpha model strains Tak-1 and Tak-2. Plant Biotechnology, 35:399-403.

♣T-AgarTrap for mature thalli

Tsuboyama-Tanaka S, Nonaka S, Kodama Y (2015) A highly efficient AgarTrap method for genetic transformation of mature thalli of the liverwort Marchantia polymorpha L. Plant Biotechnology, 32:333-336.

♣Protocol in English

Tsuboyama S, Kodama Y (2018) AgarTrap protocols on your benchtop: simple methods for Agrobacterium-mediated genetic transformation of the liverwort Marchantia polymorpha. Plant Biotechnology, 35:93-99.

♣Protocol in Japanese

田中(坪山)祥子・児玉豊(2015)”アガートラップ法:寒天に捕らえたゼニゴケを簡単に形質転換する” 植物の生長調節 50:83-86.